Pasquale Stano

Work and research

Pasquale Stano received his scientific education in chemistry at the University of Pisa, Italy. In his thesis he worked on the design and synthesis of heterotopic Shiff base ligands and relative nickel and copper supramolecular complexes, and studied the kinetics of transimmination on these substrates.

He joined Luisi's group in Zürich from February 2002, working on the liposomal drug delivery of camptothecin derivatives, in a collaboration with the Italian pharmaceutical company Sigma-Tau in Pomezia (Roma). Since 2003 he was working on the properties and reactivity of vesicles (effect of Trp-oligopeptides, turbidimetric analysis, positively charged vesicle formation), DNA condensation in reverse micelles, matrix effect and self-reproduction of vesicles, and on the AmB interaction with sterol containing vesicles. Together with Silvia Rasi, Simone Bufali, Trishool Namani, Chris Thomas, and Zhiliang Cheng, he was a member of the Luisi's laboratories for liposome research at the Department of Materials of the ETH.
In 2004 he moved to the Dept. of Biology of the University of RomaTre, where is involved in the Luisi's project dealing with the physico-chemical properties of vesicles and other compartments, and their use as cell models.

In particular, in the last three years he worked with lecithin organogel (in collaboration with the farmaceutical company Sigma-Tau), interaction between proteins and vesicles, fusion of vesicles, phosphatidic acid vesicles characterization, Cu-aminooxidase containing liposomes, pi-pi complex formation, spectroscopic characterization of random peptides and mutant proteins, preliminary studies on enzyme-mediated fatty acid vesicle formation, membrane protein reconstitution, effect of THz radiation on lipid vesicles, expression of protein in w/o emulsion, use of flow-cytometry to characterize vesicles. Currently he is involved in the Minimal Cell project (see CentroFermi) and on new approaches for understanding the origins of macromolecules in prebiotic conditions.

Recent publications

  • Stano, P.; D’Aguanno, E.; Bolz, J.; Fahr, A.; Luisi, P. L.
A remarkable self-organization process as the origin of primitive functional cells.
Angewandte Chemie, doi: 10.1002/ange.201306613

  • Chiarabelli, C.; Stano, P.; Luisi, P. L. Chemical synthetic biology: a mini-review.
Frontiers in Microbiotechnology, Ecotoxicology and Bioremediation 2013, 4, 285.

  • Rampioni, G.; Damiano, L.; Messina, M.; D’Angelo, F.; Leoni, L.; Stano, P.
Chemical communication between synthetic and natural cells: a possible experimental design.
Electronic Proceedings in Theoretical Computer Sciences 2013, 130, 14-26.

  • Cabré, E. J.; Sánchez-Gorostiaga, A.; Carrara, P.; Ropero, N.; Casanova, M.; Palacios, P.; Stano, P.; Jiménez, M.; Rivas, G.; Vicente, M.
Bacterial division proteins induce vesicle collapse and cell membrane invagination.
J. Biol. Chem. 2013, 288, 26625-26634.

  • Grotzky, A.; Altamura, E.; Adamcik, J.; Carrara, P.; Stano, P.; Mavelli, F.; Nauser, T.; Mezzenga, R.; Schlüter, A. D.; Walde, P.
Structure and enzymatic properties of molecular dendronized polymer-enzyme conjugates and their entrapment inside giant vesicles.
Langmuir 2013, 29, 10831-10840.

  • Stano, P.; D’Aguanno, E.; Carrara, P.; Fahr, A.; Luisi, P. L.
Recent advancements in synthetic cells research.
In: Advances in Artificial Life, ECAL 2013: Proceedings of the Twelfth European Conference on the Synthesis and Simulation of Living Systems (2-6 September 2013, Taormina, Italy); P. Liò, O. Miglino, G. Nicosia, S. Nolfi, M. Pavone (Eds.), MIT Press (e-book, ISBN 978-0-262-31709-2) 2013, pp. 1160-1161.

  • Mavelli, F.; Caputo, M.; Altamura, E.; Stano, P.
Stochastic simulations of minimal cell model systems.
In: Proceedings of the 12th Joint European Thermodynamics Conference, JETC 2013, M. Pilotelli and G.P. Beretta (Eds), ISBN 978-88-89252-22-2, Snoopy, Brescia, Italy, 2013, pp. 73-81.

  • Scognamiglio, V.; Stano, P.; Polticelli, F.; Antonacci, A.; Dimova Lambreva, M.; Pochetti, G.; Giardi, M. T.; Rea, G.
Design and biophysical characterization of atrazine-sensing peptides mimicking the Chlamydomonas reinhardtii plastoquinone binding niche.
Phys. Chem. Chem. Phys. 2013, 15, 13108-13115.

  • Pesiri, V.; La Rosa, P.; Stano, P.; Acconcia, F.
Identification of an estrogen receptor alpha non-covalent ubiquitin binding surface: role in 17beta-estradiol-induced transcriptional activity.
Journal of Cell Science, 2013, 126, 2577-2582.

  • Stano, P.; Souza, T. P.; Kuruma, Y.; Carrara, P.; Luisi, P. L.
Semi-synthetic minimal cells: biochemical, physical and technological aspects.
In: Synthetic Biology: Tools and Applications. H. Zhao (Ed.), Academic Press-Elsevier, Amsterdam, 2013. Pp. 261-276.

  • Calviello, L.; Stano, P.; Mavelli, F.; Luisi, P. L.; Marangoni, R.
Quasi-cellular systems: stochastic simulation analysis at nanoscale range.
BMC Bioinformatics 2013, 14(Suppl. 7), S7.

Find here the complete list of publications Pasquale Stano


Dr. Pasquale Stano

Dipartimento di Biologia
Università degli Studi di Roma Tre
Viale Marconi 446
00146 Roma

Tel.: +39.06.5733 6433; +39.06.5733 6363;
Fax: +39.06.5733 6321
e-mail : stano @